Palaeobotanical Tools /
Microtomography (CT Scanning, XTM) including Synchrotron X-ray Tomographic Microscopy (SRXTM)
Preparation and Conservation
Palynological Preparation Techniques
Cellulose Peel Technique
Photography and Scanning
Digital Cameras on the Microscope
Focus Stacking (Photography, Extended Depth of Field)
High Dynamic Range Imaging (HDR)
Transmission Electron Microscopy (TEM)
Writing, Translating and Drawing
! Chemotaxonomy and Chemometric Palaeobotany@
Glossaries, Dictionaries and Encyclopedias: Microscopy@
! R.L. Abel et al. (2012): A palaeobiologist´s guide to "virtual" micro-CT preparation. In PDF, Palaeontologia Electronica, 15.
Department of Geological Sciences (High-Resolution X-ray Computed Tomography (CT) Facility), University of Texas, Austin:
Folio. Snapshot taken by the Internet Archive´s Wayback Machine.
High-resolution X-ray CT (Computed Tomography) is a completely nondestructive technique for visualizing features in the interior of opaque solid objects, and for obtaining digital information on their 3-D geometries and properties.
What is X-ray CT? Eexcerpted and adapted from: Denison, C., Carlson, W.D., and Ketcham, R.A. 1997. Three-dimensional quantitative textural analysis of metamorphic rocks using high-resolution computed X-ray tomography: Part I. Methods and techniques. Journal of Metamorphic Geology, 15: 29-44.
J.C. Benedict (2015): A new technique to prepare hard fruits and seeds for anatomical studies. In PDF, Appl. Plant Sci., 3.
! L. Bertrand et al. (2012): Development and trends in synchrotron studies of ancient and historical materials. In PDF, Physics Reports, 519: 51-96.
! L. Bertrand et al. (2011): European research platform IPANEMA at the SOLEIL synchrotron for ancient and historical materials. In PDF, Journal of Synchrotron Radiation.
M.I. Bird et al. (2008): X-ray microtomographic imaging of charcoal. Abstract, Journal of Archaeological Science, 35: 2698-2706.
B. Blonder et al. (2012): X-ray imaging of leaf venation networks. In PDF, New Phytologist.
C. Kevin Boyce et al. (2003): CHEMICAL EVIDENCE FOR CELL WALL LIGNIFICATION AND THE EVOLUTION OF TRACHEIDS IN EARLY DEVONIAN PLANTS. Int. J. Plant Sci., 164: 691-702.
! C.R. Brodersen and A.B. Roddy (2016): New frontiers in the three-dimensional visualization of plant structure and function. American journal of botany, 103: 184-188.
! C.R. Brodersen et al. (2011): Automated analysis of three-dimensional xylem networks using high-resolution computed tomography. In PDF, New Phytologist, 191: 1168-1179.
William D. Carlson (2006): Three-dimensional imaging of earth and planetary materials. In PDF, Earth and Planetary Science Letters, 249: 133-147. Provided by the Internet Archive´s Wayback Machine.
! M.E. Collinson et al. (2016): X-ray micro-computed tomography (micro-CT) of pyrite-permineralized fruits and seeds from the London Clay Formation (Ypresian) conserved in silicone oil: a critical evaluation. Abstract.
! M.E. Collinson et al. (2012): The value of X-ray approaches in the study of the Messel fruit and seed flora. In PDF, Palaeobiodiversity and Palaeoenvironments, 92: 403-416. See also here (abstract).
! J.A. Cunningham et al. (2014): A virtual world of paleontology. Trends in Ecology & Evolution, 29: 347-357.
Charles Daghlian (Dartmouth College, Hannover, NH) and Jennifer Svitko, Paleobotanical Holdings at the Liberty Hyde Bailey Hortorium at Cornell University: Paleoclusia 3D Reconstructions. Movies from CT scans done on the Turonian fossils. Provided by the Internet Archive´s Wayback Machine. See also here (W.L. Crepet and K.C. Nixon 1998, abstract and photos).
M.L. DeVore et al. (2006): Utility of high resolution x-ray computed tomography (HRXCT) for paleobotanical studies: An example using london clay fruits and seeds. American journal of botany, 93: 1848-1851.
The Digital Morphology (part of the National Science Foundation). The Digital Morphology library is a dynamic archive of information on digital morphology and high-resolution X-ray computed tomography of biological specimens.
J.A. Dunlop et al. (2012): A minute fossil phoretic mite recovered by phasecontrast X-ray computed tomography. In PDF, Biol. Lett., 8: 457-460.
A.M.T. Elewa (2011): Computational Paleontology. Provided by Google books.
Aaron G. Filler, Department of Neurosurgery, Institute for Nerve Medicine, Santa Monica, California: The History, Development and Impact of Computed Imaging in Neurological Diagnosis and Neurosurgery: CT, MRI, and DTI (PDF file). About Magnetic Resonance Imaging, Diffusion Tensor Imaging, etc.
E.M. Friis et al. (2014): Arcellites punctatus sp. nov.: a new megaspore from the Early Cretaceous of Portugal studied using high resolution synchrotron radiation X-ray tomographic microscopy (SRXTM). In PDF, Grana, 53: 91-102. See also here.
! E.M. Friis et al. (2014): Three-dimensional visualization of fossil flowers, fruits, seeds, and other plant remains using synchrotron radiation X-ray tomographic microscopy (SRXTM): new insights into Cretaceous plant diversity. In PDF, Journal of Paleontology, 88: 684–701. See also here (abstract).
Else Marie Friis et al. (2007): Phase-contrast X-ray microtomography links Cretaceous seeds with Gnetales and Bennettitales. PDF file, Nature, 450: 549-552. See also here (abstract).
M.K. Futey et al. (2012): Arecaceae Fossil Fruits from the Paleocene of Patagonia, Argentina. In PDF, Bot. Rev.
R. Garwood and M. Sutton (2010): X-ray micro-tomography of Carboniferous stem-Dictyoptera: new insights into early insects. In PDF, Biology Letters.
R. Garwood et al. (2009): High-fidelity X-ray microtomography reconstruction of siderite-hosted Carboniferous arachnids. In PDF, Biol. Lett., 5: 841-844.
! C.T. Gee (2013): Applying microCT and 3D Visualization to Jurassic Silicified Conifer Seed Cones: a virtual advantage over thin-sectioning. In PDF, Applications in plant sciences. See also here.
C.T. Gee et al. (2003): A Miocene rodent nut cache in coastal dunes of the Lower Rhine Embayment, Germany. In PDF, Palaeontology, 46. See also here (abstract). One of the first CT applications to solve a palaeobotanical problem.
Ann Gibbons (2007): Paleontologists Get X-ray Vision. Science Vol. 318: 1546-1547.
Larry Greenemeier, Scientific American: Megavoltage CT Imaging Unlocks Fossil Mysteries. The proficiency of cancer-care computerized tomography on geologic finds.
T. Hegna et al. (2013): Not Quite Frozen in Time: Windows into the Internal Taphonomy of Fossils in Amber via MicroCT-scan Technology. Abstract.
Anette E.S. Högström et al. (2009): A pyritized lepidocoleid machaeridian (Annelida) from the Lower Devonian Hunsrück Slate, Germany. PDF file, Proc. R. Soc. B, 276: 1981-1986. This paper is exemplary in its combination of X-ray and CT of animal body fossils.
Y. Huang et al. (2012): New fossil endocarps of Sambucus (Adoxaceae) from the upper Pliocene in SW China. In PDF, Review of Palaeobotany and Palynology, 171: 152-163. Snapshot taken by the Internet Archive´s Wayback Machine.
S. Kiel et al. (2012): Fossilized digestive systems in 23 million-year-old wood-boring bivalves. In PDF.
M. Lak et al. (2008): Phase contrast X-ray synchrotron imaging: opening access to fossil inclusions in opaque amber. In PDF, Microsc. Microanal., 14, 251-259.
S. Lautenschlager, Software Sustainability Institute: A Digital (R)evolution in Palaeontology.
Karen Lee et al. (2006): Visualizing Plant Development and Gene Expression in Three Dimensions Using Optical Projection Tomography. Abstract, Plant Cell, 8(9): 2145-2156.
! A. Lukeneder (2012): Computed 3D visualisation of an extinct cephalopod using computer tomographs. In PDF, Computers & Geosciences, 45: 68-74.
! H. Mallison (2012): Digitizing Methods for Paleontology: Applications, Benefits and Limitations. In PDF.
! D. Mietchen et al. (2008): Three-dimensional Magnetic Resonance Imaging of fossils across taxa. PDF file, Biogeosciences, 5: 25-41. Fossil cones of the conifer Pararaucaria patagonica. Magnetic Resonance Imaging (MRI). See also here.
J.D. Moreau et al. (2015):
of the Histology of Leafy Axes and Male Cones of Glenrosa carentonensis sp. nov.
(Cenomanian Flints of Charente-Maritime, France)
Using Synchrotron Microtomography Linked with Palaeoecology. PloS one, 10.
Plant fossils embedded inside flint nodules.
National Center for X-ray Tomography (NXCT)
K.B. Pigg et al. (2006): VALUE OF HRXCT FOR SYSTEMATIC STUDIES OF PYRITIZED FOSSIL FRUITS. Abstract, 2006 Philadelphia Annual Meeting, Geological Society of America.
M. Pika-Biolzi et al. (2000): Industrial X-ray computed tomography applied to paleobotanical research. In PDF Rivista italiana di Paleontologia e Stratigrafia.
Palaeontology: What´s It All About?
A.R. Rees (2013): On the 3-D reconstruction of Paleozoic and Mesozoic paleobotanical problematica. Abstract.
F. Riquelme et al. (2009): Palaeometry: Non-destructive analysis of fossil materials. In PDF.
D. Schwarz et al. (2005): Neutron Tomography of Internal Structures of Vertebrate Remains: A Comparison with X-Ray Computed Tomography. Palaeontologica Electronica Volume 8, Issue 2.
! Andrew C. Scott et al. (2009): Scanning Electron Microscopy and Synchrotron Radiation X-Ray Tomographic Microscopy of 330 Million Year Old Charcoalified Seed Fern Fertile Organs. PDF file, Microsc. Microanal., 15: 166-173.
! Andrew C. Scott and Margaret E. Collinson (2003): Non-destructive multiple approaches to interpret the preservation of plant fossils: implications for calcium-rich permineralisations. PDF file, Journal of the Geological Society, 160: 857-862. See also here.
! B.J. Slater et al. (2011): Guadalupian (Middle Permian) megaspores from a permineralised peat in the Bainmedart Coal Measures, Prince Charles Mountains, Antarctica. In PDF, Review of Palaeobotany and Palynology, 167: 140-155.
! Selena Y. Smith et al. (2009): Virtual taphonomy using synchrotron tomographic microscopy reveals cryptic features and internal structure of modern and fossil plants. Abstract and free PDF (4.5 MB), PNAS, 106: 12013-12018. Excellent!
A.R.T. Spencer et al. (2013): Combined methodologies for three-dimensional reconstruction of fossil plants preserved in siderite nodules: Stephanospermum braidwoodensis nov. sp. (Medullosales) from the Mazon Creek lagerstätte. In PDF, Review of Palaeobotany and Palynology, 188: 1-17. See also here (abstract).
M. Speranza et al. (2010): Traditional and new microscopy techniques applied to the study of microscopic fungi included in amber. PDF file, In: A. Méndez-Vilas and J. Díaz (eds.): Microscopy: Science, Technology, Applications and Education. Scanning electron microscopy in backscattered electron mode, with energy dispersive X-ray spectroscopy microanalysis.
D.C. Steart et al. (2014): X-ray Synchrotron Microtomography of a silicified Jurassic Cheirolepidiaceae (Conifer) cone: histology and morphology of Pararaucaria collinsonae sp. nov. In PDF, see also here.
C. Strullu-Derrien (2014): The earliest wood and its hydraulic properties documented in c. 407-million-year-old fossils using synchrotron microtomography. Abstract, Botanical Journal of the Linnean Society, 175: 423-437.
! G.W. Stull et al. (2016): Revision of Icacinaceae from the Early Eocene London Clay flora based on X-ray micro-CT. In PDF (26 MB), NRC Research Press. See also here
Wolfgang H. Stuppy et al. (2003): Three-dimensional analysis of plant structure using high-resolution X-ray computed tomography. PDF file, Trends in Plant Science, 8.
! M.D. Sutton et al. (2012): SPIERS and VAXML; A software toolkit for tomographic visualisation and a format for virtual specimen interchange. In PDF, Palaeontologia Electronica, 15.
! M.D. Sutton (2008): Tomographic techniques for the study of exceptionally preserved fossils. PDF file, Proc. R. Soc. B, 275: 1587-1593.
P. Tafforeau et al. (2007): Nature of laminations and mineralization in rhinoceros enamel using histology and X-ray synchrotron microtomography: Potential implications for palaeoenvironmental isotopic studies. Abstract, Palaeogeography, Palaeoclimatology, Palaeoecology, 246: 206-227.
T. van der Niet et al. (2010): Three-dimensional geometric morphometrics for studying floral shape variation. In PDF, Trends in Plant Science, 15.
S.-J. Wang et al. (2017): Anatomically preserved "strobili" and leaves from the Permian of China (Dorsalistachyaceae, fam. nov.) broaden knowledge of Noeggerathiales and constrain their possible taxonomic affinities. In PDF, Am. J. Bot., 104: 127-149.
Synchrotron Based Scanning Transmission X-ray Microscopy and Microspectroscopy
(C-, N-, O-XANES).
Snapshot provided by the Internet Archive´s Wayback Machine.
! M.W. Westneat (2008):
in biological structure, function, and physiology using synchrotron X-ray imaging. In PDF,
Annu. Rev. Physiol., 70: 119-142.
This expired link is available through the Internet Archive´s Wayback Machine.
Wikipedia, the free encyclopedia:
! Synchrotron X-ray Tomographic Microscopy.
Category:X-ray computed tomography.
Tomografie (in German).
Computertomographie (in German).
Kategorie:Tomografie (in German).
! A. Ziegler et al. (2010):
and challenges for digital morphology. In PDF,
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