Home / Palaeobotanical Tools / Fluorescence Microscopy and Fluorescence Microspectroscopy

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High Dynamic Range Imaging (HDR) Image Processing Imaging Fossils Using UV-Light (Black-Light Photography) X-ray Transmission Electron Microscopy (TEM) Microtomography (CT Scanning, XTM) including Synchrotron X-ray Tomographic Microscopy (SRXTM) Raman Spectroscopy Writing, Translating and Drawing Geostatistics Making Thin Sections ! Three-Dimensionally Preserved Plant Compression Fossils@

! L. Barlow et al. (2020): Laser-stimulated fluorescence reveals unseen details in fossils from the Solnhofen Limestone (Upper Jurassic, Bavaria, Germany). In PDF, ResearchSquare.com.

D.E. Quiroz Cabascango (2023): Plant Macrofossils from the Aftermath of the End-Triassic Extinction, Skåne, Southern Sweden. Free access, Thesis, Department of Earth Sciences, Uppsala University.
Note figure 11: Epidermal cells and stomata of conifers of Brachyphyllum sp. under fluorescence microscopy.

P. Debertolis and N. Earl (2014): Forensic Imaging in Anthropology. In PDF, Human And Social Sciences at the Common Conference, 2014.

A.R. Falk et al. (2016): Laser Fluorescence Illuminates the Soft Tissue and Life Habits of the Early Cretaceous Bird Confuciusornis. Open access, PLoS ONE, 11: e0167284.

M. Frese et al. (2017): Imaging of Jurassic fossils from the Talbragar Fish Bed using fluorescence, photoluminescence, and elemental and mineralogical mapping. Open access, PLoS ONE, 12: e0179029.
"... Closer inspection of a plant leaf (Pentoxylon australicum White, 1981) establishes fluorescence as a useful tool for the visualisation of anatomical details that are difficult to see under normal light conditions".

! C. Haug et al. (2009): New Methods to Document Fossils from Lithographic Limestones of Southern Germany and Lebanon. PDF file, Palaeontologia Electronica, 12.

! T.G. Kaye et al. (2015): Laser-Stimulated Fluorescence in Paleontology. Open access, PLoS ONE 10(5): e0125923.

! H. Kerp and B. Bomfleur (2011): Photography of plant fossils - New techniques, old tricks. In PDF, Review of Palaeobotany and Palynology, 166: 117-151.
See also here (abstract with reduced figures and plates).

! J.W. Lichtman and J.A. Conchello (2005): Fluorescence microscopy. In PDF. Nature methods, 2: 910–919. See likewise here.

! L. Luthardt et al. (2023): Cycadodendron galtieri gen. nov. et sp. nov.: An Early Permian Gymnosperm Stem with Cycadalean Affinity. Free access, International Journal of Plant Sciences, 184.
Note figure 10: Details of cycad-specific stem-anatomical features.
"... Cycadodendron galtieri gen. nov. et sp. nov. represents a petrified cycad stem of early Permian age providing the oldest-known evidence of cycad anatomy.
[...] The broad anatomical similarities of C. galtieri with other fossil and extant cycads demonstrate the early evolution of various cycad-specific anatomical features in the lower Permian ..."

D.E. Quiroz Cabascango (2023): Plant Macrofossils from the Aftermath of the End-Triassic Extinction, Skåne, Southern Sweden. Free access, Thesis, Department of Earth Sciences, Uppsala University.

! A. Richards (2010): Reflected Ultraviolet Imaging for Forensics Applications. In PDF, Santa Barbara, 2010.

C.S. Shi et al. (2013): Characterization of the stem anatomy of the Eocene fern Dennstaedtiopsis aerenchymata (Dennstaedtiaceae) by use of confocal laser scanning microscopy. Free access, American Journal of Botany, 100: 1626–1640.

M. Slodownik et al. (2023): Chasing a ghost through Gondwana's history–the fossil record of the 'seed fern' Komlopteris. Open access, Australasian Systematic Botany Society Newsletter, 196: 9-12.
! Note figure 1B, C: UV fluorescence image of a frond.

M. Slodownik et al. (2023): Komlopteris: A persistent lineage of post-Triassic corystosperms in Gondwana. Free access, Review of Palaeobotany and Palynology, 317.
! Note figure Plate 1, fig. 3, 4: UV- fluorescence photographs.
! Plate 2: UV fluorescence images.

! M.R. Stoneman et al. (2024): Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants. Open access, Communications Biology, 7.
"... In this work, we utilize two-photon fluorescence microspectroscopy to spatially and spectrally resolve the fluorescence emitted by amber-embedded plants, leaf compressions, and silicified wood
[...] This research opens doors to exploring ancient ecosystems and understanding the ecological roles of fluorescence in plants throughout time. ..."

Wikipedia, the free encyclopedia:
Fluorescence.
Category:Fluorescence.
Category:Fluorescence techniques.
Category:Optical microscopy techniques.
! Fluorescence microscope.

! R. Williams and G. Williams (2005): Ultraviolet, Infrared & Fluorescence Photography. In PDF, Medical and Scientific Photography, 2005.

! K. Wolkenstein and G. Arp (2021): Taxon- and senescence-specific fluorescence of colored leaves from the Pliocene Willershausen Lagerstätte, Germany. Open access, PalZ.